| Pseudomonas aeruginosa produces large array of extracellular toxins that play a crucial role in virulence potential. In this study, the virulence profile of 35 clinical isolates of P. aeruginosa distinctly recovered from pneumonic cattle, mastitic milk samples, and wound infections was determined using phenotypic and genotypic methods. The overall incidence rates from pneumonic cattle, mastitic milk samples, and wound infections were 6%, 25% and 13.3%, respectively with a total incidence of

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... %. Recovered isolates were then assayed for their enzymatic activity and the results revealed that out of these 35 isolates, 28 isolates produced haemolytic activity on blood agar plates, 27 isolates showed lecithinase enzyme activity on egg yolk agar media and 12 isolates showed proteolytic enzyme activity on brain heart infusion agar medium supplied with gelatin. Three virulent genes (las B, tox A and exoS) were screened by polymerase chain reaction (PCR) and the results showed that las B, tox A and exoS genes were amplified from 14.7%, 8.57%, and 17.14% of tested isolates, respectively. In conclusion, the present studies confirmed previous observations that the virulence of P. aeruginosa is multifactorial; however, the results indicate that certain exoproducts may play a more important role in certain types of infection.