Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma

Ryoji Kitamura, Takeshi Toyoshima, Hideaki Tanaka, Shintaro Kawano, Takahiro Kiyosue, Ryota Matsubara, Yuichi Goto, Mitsuhiro Hirano, Kazunari Oobu, Seiji Nakamura
2012 Journal of Cancer Research and Clinical Oncology  
Purpose The aim of this study was to conWrm the expression proWle of cytokeratin (CK)17 in comparison with that of CK13 in oral squamous cell carcinoma (OSCC) and leukoplakia and to clarify an association of CK17 with the OSCC diVerentiation. Materials The expression of CK17 and CK13 was immunohistochemically examined in 105 patients with OSCC and 108 patients with leukoplakia. A correlation of CK expression with clinicopathological variables was carried out. The over-expression levels of CK17
more » ... RNA were analyzed by real-time RT-PCR in 5 OSCC cell lines (HSC-2, HSC-3, SAS, SQUU-A, SQUU-B). Results CK17 and CK13 were detected in 101 (96.2 %) and three (2.9 %) of the 105 OSCCs, respectively. CK17 was signiWcantly expressed in well-diVerentiated OSCC compared to moderately/poorly diVerentiated OSCC (p < 0.01). As detected in 19 of the 34 dysplastic leukoplakias (55.9 %) and 36 of the 74 hyperplastic leukoplakias (48.6 %), CK17 was signiWcantly expressed in dysplastic leukoplakias (p < 0.01). As detected in 11 of the 34 dysplastic (32.4 %) and 52 of the 74 hyperplastic leukoplakias (70.3 %), CK13 was signiWcantly expressed in hyperplastic leukoplakias (p < 0.01). The relative expression of CK17 mRNA in HSC-2 was signiWcantly higher than in HSC-3 and SAS (p < 0.05). Moreover, the relative expression of CK17 mRNA in SQUU-A was signiWcantly higher than in SQUU-B (p < 0.05). Conclusion CK17 expression could be associated with the diVerentiation and the malignancy of OSCC. A combination pattern of CK17/CK13 might be a suitable marker of malignant transformation.
doi:10.1007/s00432-012-1202-6 pmid:22466643 pmcid:PMC3397222 fatcat:zywv7tqyajeb5hakdmzoq2kqse