大会講演要旨

2001 Nippon Nogeikagaku-kaishi  
Synthetic studies on carbohydrates and glycoconjugates are of growing importance because a variety of synthetic substrates, ligands, and inhibitors are expected to become powerful tools both for investigating biological roles of glycoconjugates and for creating novel class of glycomimics and glycomaterials in medical and industrial fields. Therefore, development of efficient and versatile synthetic methods is strongly required recently. Enzymatic synthesis is an excellent and potent method for
more » ... he preparation of glycoconjugates as well as the conventional chemical synthesis. Actually, the use of glycosyltransferases in the synthetic schemes has accelerated practical synthesis of oligosaccharides and related compounds. However, there is no systematic approach for establishing "automated glycosynthesis" based on the combined chemical and enzymatic strategy. Recently, we found that water-soluble glycopolymers bearing multivalent sugar-branches become excellent glycosyl acceptor substrates of glycosyltransferases. It was demonstrated that the polymeric sugar-cluster effect drastically enhanced the affinity of enzymes with glycosyl acceptors. Moreover, it was also suggested that this type of soluble polymer substrates can be analyzed by using common spectroscopic methods and purified by the simple gel filtration chromatography to afford highly glycosylated products in high yields. When some designed linkers that can be recognized by specific enzymes are involved in the glycopolymers, the products are easily released from the polymer supports by treating them with the designated enzymes after the sugar elongation reactions. On the other hand, we have established an efficient recycling system for the engineered glycosynthesis based on the glycosyltransferases. Thus, some glycosyltransferases
doi:10.1271/nogeikagaku1924.75.sup_522 fatcat:64pv7iis65cuvoceywqxafl66q