Properties of Maedi Nucleic Acid and the Presence of Ribonucleic Acid- and Deoxyribonucleic Acid-Dependent Deoxyribonucleic Acid Polymerase in the Virions

Fu Hai Lin, Halldor Thormar
1972 Journal of Virology  
Maedi virus contains a ribonucleic acid (RNA) which can be resolved into three major components, namely, 62S, 33S, and 13S, by sucrose gradient centrifugation. The presence of RNAand deoxyribonucleic acid (DNA)-dependent DNA polymerase in virions of maedi virus was demonstrated. The enzyme product could be converted into acid-soluble form by pancreatic deoxyribonuclease, but was resistant to digestion by pancreatic ribonuclease and to hydrolysis by NaOH. ml of maintenance medium containing 10
more » ... ium containing 10 ,uCi of 3Huridine (25 Ci/mmole) per ml or 10 ,Ci of 3H-uridine plus 1 jACi of 14C-methyl-thymidine (20 to 30 mCi/mmole) per ml. Thereafter, 30 ml more of the same medium was added to the cultures which were incubated at 37 C. The infectious fluid was harvested 228 on May 9, 2020 by guest
doi:10.1128/jvi.10.2.228-233.1972 fatcat:utbzghs6jjb6jhpinazvi3evc4