DNA Barcoding for Differentiating the 11 Varieties of Musa Species

Saifuldeen Ahmed Hasan Saifuldeen Ahmed Hasan
2018 International Journal of Agricultural Science and Research  
The present investigation was aimed to evaluate the ITS 2 as a mini -barcode for analyzing the phylogenetic relationship among the 11 varieties of Musa species. DNA barcoding was performed by ITS sequencing employing the ITS 2 primer. All the resultant sequences were submitted to NCBI database to attain GenBank accession numbers. MEGA 6.06 software was employed for ITS sequences to determine the phylogenetic relationship among the 11 varieties of Musaspp by Neighbor-Joining method. The primer
more » ... S 2 generated a mini-barcode for all the 11 varieties of Musa spp. and the GenBank accession numbers were obtained for all the sequences from the NCBI database. The 11 varieties of Musa spp. clustered into 3 groups in the phylogenetic tree.
doi:10.24247/ijasraug201816 fatcat:z747n7kv4vgife37iuhk3rkyxa