A model to measure lymphatic drainage from the eye

Minhui Kim, Miles G. Johnston, Neeru Gupta, Sara Moore, Yeni H. Yücel
2011 Experimental Eye Research  
Intraocular pressure (IOP) is the most important risk factor for glaucoma development and progression. Most anti-glaucoma treatments aim to lower IOP by enhancing aqueous humor drainage from the eye. Aqueous humor drainage occurs via well-characterized trabecular meshwork (TM) and uveoscleral (UVS) pathways, and the recently described ciliary lymphatics. The relative contribution of the lymphatic pathway to aqueous drainage is not known. We developed a sheep model to quantitatively assess
more » ... tic drainage along with TM and UVS outflows. Following intracameral injection of 125 I-bovine serum albumin (BSA), lymph and blood samples were continuously collected. Lymphatic and TM drainage were quantitatively assessed by measuring 125 I-BSA recovery. This quantitative sheep model enables assessment of relative contributions of lymphatic drainage (1.64% ± 0.89%), TM (68.86% ± 9.27%) and UVS outflows (19.87% ± 5.59%), and may help to better understand the effects of glaucoma agents on outflow pathways. iii Dedication This thesis is dedicated to my parents and little brother for their love, endless support and encouragement. iv
doi:10.1016/j.exer.2011.07.006 pmid:21810424 fatcat:utft3lpt4nfl7iuuqlmpcb6vhy