Characterization of the, Antimicrobial, Antioxidant Activity of Proteins from Prosopis cineraria Leaves

Mayank Kulshreshtha, Karuna Shanker Shukla, Garima Tiwari, Manjul Pratap Singh
2019 Pharmacognosy Communications  
Introdutction:The present study was designed to screen out the different proteins from Prosopis cineraria (Leaves), known as Shammi (Family Fabaceae) and characterize them by IR, NMR and mass spectroscopy and evaluation of their antimicrobial potential against various microbial species. Mehtods: Different extracts of leaf were treated with different reagents for the presence and absence of metabolites. Antimicrobial effect of the proteins were explored against Staphylococcus aureus, Pseudomonas
more » ... aeruginosa and Aspergillus tubingensis. The disc diffusion method was used for deter mination of antimicrobial activity. Analytical analysis included IR, NMR and Mass spectroscopy. Reults: Phytochemical analysis of different extracts showed the presence of different primary and secondary metabolites The range of zone of inhibition were found to be from 1.4±0.01mm to 14.2±0.18 mm. The maximum antimicrobial activity was found for 50 KDa protein against Pseudomonas aeruginosa. Identification of carbohydrate, alkaloids, flavonoids, tannins, proteins, terpenoids etc was achieved by standard chemical tests. [30] [31] [32] Screening of different proteins from ethanolic extract of P. cineraria through SDSPAGE electrophoresis Resolving gel, stacking gel were prepared and sample preparation was done by 20-40 micro grams total protein per mini-gel loaded in wells
doi:10.5530/pc.2019.2.12 fatcat:iyhgqvh2rzd6fb2dcpyefc22ti