Detection of Different Iranian Isolates of Bovine Herpes Virus Type-I (BHV-I) Using Polymerase Chain Reaction

Kargar Moakhar, I Ghorashi'l, S Sadeghi, J Morshedi, D ' Masoudi, S Pourbakhsh
2003 unpublished
Based on primers from gl glycoprotein, a polymerase chain reaction (PCR) assay was optimized for detection of bovine herpes virus type 1 (BHV-1). A 468bp DNA fragment ofnine isolates was amplified. The PCR products were detected by ethidium bromide staining after gel electrophoresis and confirmed by sequencing. The nucIeotide sequence alignments revealed a highly conserved region in gI gene within the isolates. Results suggest that PCR can be used as a reliable diagnostic tool for rapid detection of BHV-1 infections.