Directly acting fibrinolytic enzyme is found to be superior therapy for treatment of cardiovascular diseases. Hence, thereis a need of a novel and highly potent fibrinolytic agent which can directly degrade fibrin clot in lesser time withimproved specificity and overcome shortcomings of haemorrhage. Amongst various sources, microbial fibrinolyticenzymes have attracted attention of research community so the present study aimed to isolate fibrinolytic enzymeproducingbacteria from different soil

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... mples. Caseinolytic activity and clot lyses activity of the crude enzyme producedby these isolates were determined. All together six caseinolytic organisms were isolated, 3 of them produced fibrinolyticprotease which would lyse fibrin and whole blood clot. Caseinolytic activity in the crude enzyme preparation fromColony C₄ was detected to be 78.2 U/ml and was able to lyse 85.54% of whole blood clot in 90 mins. Colony 4 may beof potential interest for biotechnological use as it produces fibrinolytic proteases that degraded fibrin clot and the wholeblood clot, to the maximum.