Plant compounds that induce polychlorinated biphenyl biodegradation by Arthrobacter sp. strain B1B

E S Gilbert, D E Crowley
1997 Applied and Environmental Microbiology  
Plant compounds that induced Arthrobacter sp. strain B1B to cometabolize polychlorinated biphenyls (PCBs) were identified by a screening assay based on the formation of a 4,4-dichlorobiphenyl ring fission product. A chemical component of spearmint (Mentha spicata), l-carvone, induced Arthrobacter sp. strain B1B to cometabolize Aroclor 1242, resulting in significant degradation of 26 peaks in the mixture, including selected tetraand pentachlorobiphenyls. Evidence for PCB biodegradation included
more » ... gradation included peak disappearance, formation of a phenylhexadienoate ring fission product, and chlorobenzoate accumulation in the culture supernatant. Carvone was not utilized as a growth substrate and was toxic at concentrations of greater than 500 mg liter ؊1 . Several compounds structurally related to l-carvone, including limonene, p-cymene, and isoprene, also induced cometabolism of PCBs by Arthrobacer sp. strain B1B. A structure-activity analysis showed that chemicals with an unsaturated p-menthane structural motif promoted the strongest cometabolism activity. These data suggest that certain plant-derived terpenoids may be useful for promoting enhanced rates of PCB biodegradation by soil bacteria.
doi:10.1128/aem.63.5.1933-1938.1997 fatcat:d4hozmwd2bcmzaxf4wrsbs3xiy