Hepatitis A virus polyprotein processing by Escherichia coli proteases

Rosa M. Pintó, Juan F. González-Dankaart, Santiago Caballero, Ke-Jian Guo, Susana Guix, Albert Bosch, Gloria Sánchez, Enric Ribes
2002 Journal of General Virology  
Hepatitis A virus (HAV) encodes a single polyprotein, which is post-translationally processed. This processing represents an essential step in capsid formation. The virus possesses only one protease, 3C, responsible for all cleavages, except for that at the VP1/2A junction region, which is processed by cellular proteases. In this study, data demonstrates that HAV polyprotein processing by Escherichia coli protease(s) leads to the formation of particulate structures. P3 polyprotein processing in
more » ... otein processing in E. coli is not dependent on an active 3C protease : the same processing pattern is observed with wild-type 3C or with several 3C mutants. However, this processing pattern is temperature-dependant, since it differs at 37 or 42 mC. The bacterial protease(s) cleave scissile bonds other than those of HAV ; this contributes to the low efficiency of particle formation.
doi:10.1099/0022-1317-83-2-359 pmid:11807229 fatcat:fz3f4cgnyreovfwtewosxcxcuy