Original Article Benzaldehyde levofloxacin schiff baseinduced apoptosis of human hepatocarcinoma cells

Hong-Xia Liang, Yuan-Yuan Fan, Ying Zhang, Chao-Shen Huangfu, Guo-Qiang Hu, Bin Liu
2016 Int J Clin Exp Med   unpublished
To study the effect of (S)-1, 8-(2-methyl phosphate ethoxy)-6-fluorine-7-(4-methyl-pi-perazine-1-base)-3-[S-benzyls-based-4-(for nitrobenzene methylene group amino)-1, 2, 4-all triazole-3 base]-quinoline (1-H)-4-ketone (M18) on apoptosis of hepatocarcinoma SMMC-7721 cells in vitro. Different concentrations of M18 at different time were used to treat SMMC-7721 cells, human breast cancer MB-231 cells, human colon cancer HCT-116 cells, human hepatocarcinoma HEPG-2 cells, mouse bone marrow
more » ... one marrow mesenchymal stem cells (BMSCs) in vitro. The inhibition effects of M18 on cell proliferation were examined by MTT assay. Cell apoptosis was determined using Hoechst 33258 fluorescence staining and TUNEL method. Mitochondrial membrane potential (Δψm) was measured using a high content screening image system. Protein expression of caspase-3, p53 and cytochrome C was detected with Western blot analysis. Treatment with M18 (4~32 μmol·L-1) potently inhibited the proliferation of the cancer cells in time-and dose-dependent manners (the IC50 value at 24 h in SMMC-7721 cells, MB-231 cells, HCT-116 cells and HEPG-2 cells was 8.65 μmol·L-1 , 9.37 μmol·L-1 , 12.74 μmol·L-1 and 9.40 μmol·L-1 , respectively). In contrast, M18 had weak cytotoxicity against BMSCs with IC50 value of 38.96 μmol·L-1. Levofloxacin had weak cytotoxicity against SMMC-7721 cells with IC50 value of 735.10 μmol·L-1. Treatment of SMMC-7721 cells with different concentrations of M18 for 24 h increased the percentage of the apoptosis cells (P < 0.05) and decreased the mitochondrial membrane potential. In addition, M18 increased protein expression of p53, caspase-3 and the cleaved activated forms of caspase-3 in SMMC-7721 cells. Treatment of SMMC-7721 cells with M18 significantly increased cytochrome C in the cytosol, and decreased cytochrome C in the mitochondrial compartment. The mitochondrial-dependent pathways are involved in M18 induction of apoptosis of SMMC-7721 cells.