Plastic Embedding and Sectioning of Plant Tissues

Tie Liu
2012 Bio-protocol  
Plastic (resin) embedding provides exclusively improvements to cellular definition compared to paraffin embedding. The combination of strongly cross-linking paraformaldehyde with glutaraldehyde and post fixed with OsO4 is the fixative of choice for high-resolution light microscopy and electron microscopy. For this reason, this method is an ideal tool for visualizing plant cellular morphology and phenotype.
doi:10.21769/bioprotoc.296 fatcat:srrjhn2gmbf43pr5lztueea3ne