A glance at DNA microarray technology and applications

Amir Ata Saei, Yadollah Omidi
2011 BioImpacts  
Because of huge impacts of "OMICS" technologies in life sciences, many researchers aim to implement such high throughput approach to address cellular and/or molecular functions in response to any influential intervention in genomics, proteomics, or metabolomics levels. However, in many cases, use of such technologies often encounters some cybernetic difficulties in terms of knowledge extraction from a bunch of data using related softwares. In fact, there is little guidance upon data mining for
more » ... ovices. The main goal of this article is to provide a brief review on different steps of microarray data handling and mining for novices and at last to introduce different PC and/or web-based softwares that can be used in preprocessing and/or data mining of microarray data. To pursue such aim, recently published papers and microarray softwares were reviewed. It was found that defining the true place of the genes in cell networks is the main phase in our understanding of programming and functioning of living cells. This can be obtained with global/selected gene expression profiling. Studying the regulation patterns of genes in groups, using clustering and classification methods helps us understand different pathways in the cell, their functions, regulations and the way one component in the system affects the other one. These networks can act as starting points for data mining and hypothesis generation, helping us reverse engineer.
doi:10.5681/bi.2011.011 pmid:23678411 pmcid:PMC3648957 fatcat:4ywgkf6i2zaxtg7z6lvzvhfjn4