Developmental and hormonal regulation of type II DNA topoisomerase in rat testis

R. Bakshi
2001 Journal of Molecular Endocrinology  
Type II DNA topoisomerase (topo II) is required for diverse biological functions including DNA replication, maintenance of genome stability, chromosome segregation and chromosome condensation. While the identity of topo II in rodent testis has been established, the regulation of topo II expression during the development of the postnatal testis and gametogenesis is unclear. Here, we report that rat testis topo II is developmentally and hormonally regulated. Topo II mRNA levels peaked prior to
more » ... peaked prior to the onset of puberty, declined sharply thereafter and stabilized in adult testis. In contrast, the topo II enzyme content was lower in prepubertal testis but increased after the onset of puberty. Topo II was expressed in a cell-specific manner within germ cells, being detected only in pachytene spermatocytes. While testosterone markedly increased topo II mRNA levels in prepubertal testis, continued treatment failed to enhance topo II mRNA above postpubertal control levels. The
doi:10.1677/jme.0.0260193 pmid:11357056 fatcat:5uuddzar7ngt3iqwhf2o4iorl4