Effect of tonsillectomy on specific immune responses for nontypeable Haemophilus influenzae

Masashi Suzuki, Satoru Kodama, Naoko Sakamoto, Goro Mogi
1999 Stomato-pharyngology  
The effect of tonsillectomy on specific immune responses for nontypeable Haemophilus influenzae was investigated. Tonsil tissues, nasopharyngeal secretions, and epithelial cells of palatine tonsils were obtained and cultured from 32 patients with chronic tonsillitis or tonsillar hypertrophy (mean age, 18; range, 3 to 66) who underwent tonsillectomy. Mononuclear cells were isolated from the tonsils, and the numbers of P6-specific antibody secreting cells were determined by ELISPOT assay. Before
more » ... nd 1 month after tonsillectomy, we determined antibody titers against P6 in nasopharyngeal secretions and sera by ELISA, concentrations of cytokines in nasopharyngeal secretions by using ELISA kits, and incidence of P6-specific intracellular cytokines producing CD4+T cells in peripheral blood by flow cytometry. Nontypeable Haemophilus influenzae was cultured frequently, especially in young children, from the epithelial cells and from tonsil tissues and nasopharyngeal secretions. The presence or absence of nontypeable Haemophilus influenzae inside the epithelial cell did not exert particular influence on the number of P6-specific antibody secreting cells of the tonsil. The incidence of nontypeable Haemophilus influenzae detected in the nasopharyngeal secretion decreased after the tonsillectomy. However, significant differences were not observed in P6-specific antibody titers in the nasopharyngeal secretion and sera, in concentrations of cytokines in the nasopharyngeal secretion, or in the ratio of Thl and Th2 phenotypes of CD4+T cells that produce P6-specific intracellular cytokines in peripheral blood. These findings suggest that a tonsillectomy may be effective in eliminating nontypeable Haemophilus influenzae from the upper aerodigestive tract and that it may not result in depressed P6specific immune responses.
doi:10.14821/stomatopharyngology1989.11.277 fatcat:3f5jncz33fa5tcxxpbpea7id2q