Mapping the human DC lineage through the integration of high-dimensional techniques

Peter See, Charles-Antoine Dutertre, Jinmiao Chen, Patrick Günther, Naomi McGovern, Sergio Erdal Irac, Merry Gunawan, Marc Beyer, Kristian Händler, Kaibo Duan, Hermi Rizal Bin Sumatoh, Nicolas Ruffin (+24 others)
2017 Science  
max) Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally-specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here we combine two high-dimensional technologies, single-cell mRNA sequencing and Cytometry by Time-of-Flight (CyTOF), to identify human blood CD123 + CD33 + CD45RA + DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC
more » ... lasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed sub-populations including one early uncommitted CD123 high pre-DC subset and two CD45RA + CD123 low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting. One Sentence Summary: Mapping the human dendritic cell lineage
doi:10.1126/science.aag3009 pmid:28473638 fatcat:d7qakxhmzvglblbll2w25xtxaq