Generation of Recombinant N-Linked Glycoproteins in E. coli [chapter]

Benjamin Strutton, Stephen R. P. Jaffé, Jagroop Pandhal, Phillip C. Wright
2017 Msphere  
The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E. coli, utilizing Campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. To ensure a successful outcome, various methods will be highlighted that
more » ... e highlighted that can confirm glycoprotein production to a high degree of confidence, including the gold standard of mass spectrometry analysis.
doi:10.1007/978-1-4939-6887-9_15 pmid:28470609 fatcat:evwj4xmitbh57eopdcwyulep4e