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Avaliação de DNA polimerases em ensaios de amplificação de microssatélites através do PowerPlex®16 BIO System
2014
BBR - Biochemistry and Biotechnology Reports
This study aimed to compare several DNA polymerases in order to optimize costs of microsatellite amplification reaction through the PowerPlex®16 BIO System. Three different polymerase brands have been used: (i) Paq5000 DNA Polymerase (Stratagene) and (ii) Platinum® Taq DNA Polymerase (Invitrogen), which have low commercial price; and (iii) Advantage 2 Polymerase Mix (Clontech), a DNA polymerase with high commercial price. Herein, neither samples nor positive control amplified in the reactions
doi:10.5433/2316-5200.2014v3n2p1
fatcat:ggdimqncvrbqninchnjyejuwtu