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We reported a case with increased serum immunoreactive insulin (IRI) and Cpeptide immunoreactivity (CPR). The molar ratio of IRI to CPR was also increased. The propositus was diabetic with background retinopathy and neuropathy. No antibody to insulin or insulin receptor was detected in his serum and his insulin resistance was not so remarkable. When the serum was fractionated by gel filtration, about 90% of total IRI was recovered in the fraction where biosynthetic human proinsulin was eluted.doi:10.1507/endocrj1954.36.545 pmid:2684621 fatcat:baku6e444ba47dqt6okf4nukb4