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The dense network of interconnected cellular signalling responses that are quantifiable in peripheral immune cells provides a wealth of actionable immunological insights. Although high-throughput single-cell profiling techniques, including polychromatic flow and mass cytometry, have matured to a point that enables detailed immune profiling of patients in numerous clinical settings, the limited cohort size and high dimensionality of data increase the possibility of false-positive discoveries anddoi:10.1038/s42256-020-00232-8 pmid:33294774 pmcid:PMC7720904 fatcat:kptdfh4mgjahjojfccqrhhbvbi