Two-photon laser scanning microscopy with electrowetting-based prism scanning

Omkar D. Supekar, Baris N. Ozbay, Mo Zohrabi, Philip D. Nystrom, Gregory L. Futia, Diego Restrepo, Emily A. Gibson, Juliet T. Gopinath, Victor M. Bright
2017 Biomedical Optics Express  
Laser scanners are an integral part of high resolution biomedical imaging systems such as confocal or 2-photon excitation (2PE) microscopes. In this work, we demonstrate the utility of electrowetting on dielectric (EWOD) prisms as a lateral laser-scanning element integrated in a conventional 2PE microscope. To the best of our knowledge, this is the first such demonstration for EWOD prisms. EWOD devices provide a transmissive, low power consuming, and compact alternative to conventional adaptive
more » ... optics, and hence this technology has tremendous potential. We demonstrate 2PE microscope imaging of cultured mouse hippocampal neurons with a FOV of 130 × 130 μm 2 using EWOD prism scanning. In addition, we show simulations of the optical system with the EWOD prism, to evaluate the effect of propagating a Gaussian beam through the EWOD prism on the imaging quality. Based on the simulation results a beam size of 0.91 mm full width half max was chosen to conduct the imaging experiments, resulting in a numerical aperture of 0.17 of the imaging system.
doi:10.1364/boe.8.005412 pmid:29296477 pmcid:PMC5745092 fatcat:lcsorgf4ubbnngfascacro36ou