Contemplating 3-Hydroxypropionic Acid Biosynthesis in Klebsiella pneumoniae

Ying Li, Pingfang Tian
2015 Indian Journal of Microbiology  
3-Hydroxypropionic acid (3-HP) is a commercially valuable platform chemical from which an array of C 3 compounds can be generated. Klebsiella pneumoniae has been considered a promising species for biological production of 3-HP. Despite a wealth of reports related to 3-HP biosynthesis in K. pneumoniae, its commercialization is still in infancy. The major hurdle hindering 3-HP overproduction lies in the poor understanding of glycerol dissimilation in K. pneumoniae. To surmount this problem, this
more » ... eview aims to portray a picture of 3-HP biosynthesis, involving 3-HP-synthesizing strains, biochemical attributes, metabolic pathways and key enzymes. Inspired by the state-of-the-art advances in metabolic engineering and synthetic biology, here we advocate protocols for overproducing 3-HP in K. pneumoniae. These protocols range from cofactor regeneration, alleviation of metabolite toxicity, genome editing, remodeling of transport system, to carbon flux partition via logic gate. The feasibility for these protocols was also discussed.
doi:10.1007/s12088-015-0513-0 pmid:25805899 pmcid:PMC4363255 fatcat:csonomlqvngwdamsefp3i3q7ze