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Probing the Function of the Invariant Glutamyl Residue 312 in Spinach Ferredoxin-NADP+Reductase
1998
Journal of Biological Chemistry
Ferredoxin-NADP ؉ reductase, the prototype of a large family of structurally related flavoenzymes, pairs single electrons carried by ferredoxin I and transfers them as a hydride to NADP ؉ . Four mutants of the enzyme, in which Glu-312 was replaced with Asp, Gln, Leu, and Ala to probe the role of the residue charge, size, and polarity in the enzyme activity, have been heterologously expressed, purified, and characterized through steadystate, rapid kinetic studies, ligand-binding experiments, and
doi:10.1074/jbc.273.51.34008
pmid:9852055
fatcat:vu7tv37m7ne3bgpgfrc5u7aabq