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Immunofluorescence study of actin, acrosin, dynein, tubulin and hyaluronidase and their impact on in-vitro fertilization
1992
Human Reproduction
Using polyclonal antibodies, the distribution of actin, acrosin, dynein, tubulin and hyaluronidase has been examined by indirect immunofluorescence in sperm preparations from fertile donors and in-vitro fertilization (TVF) patients. After recording sperm parameters in native semen, spermatozoa were washed free of seminal plasma using either the swimup or the Percoll filtration technique. Prior to insemination, aliquots of the washed sperm suspensions were prepared for antibody staining.
doi:10.1093/oxfordjournals.humrep.a137747
pmid:1386853
fatcat:g36kyypfxjgmhkoimrnjgtqanm