Schistosome egg antigen(s) presentation and regulatory activity by macrophages isolated from vigorous or immunomodulated liver granulomas of Schistosoma mansoni-infected mice
Journal of Immunology
Previously, it was shown that macrophages isolated from liver granulomas of Schistosoma mansoni-infected mice constituted about 30% of the granuloma cell population, were highly activated, and more than 50% displayed H-2 I region-associated antigens. Moreover, the degree of macrophage maturation/activation correlated with the intensity of the granulomatous response. Thus, inflammatory macrophages from the vigorous granulomas of acutely infected mice (VigGrM phi) displayed greater phagocytic and
... tumoricidal activity than did M phi from the T suppressor cell-modulated granulomas (ModGrM phi) of chronically infected animals. No difference was seen, however, in the percentage of Ia-bearing M phi isolated from vigorous or modulated liver granulomas. Presently, accessory activity of GrM phi was assayed by reconstitution of the proliferative responses of M phi-depleted lymphocytes. Both VigGrM phi and ModGrM phi were able to reconstitute the Con-A induced proliferation of normal thymoctyes. Moreover, both types of GrM phi could reconstitute parasite egg antigen-specific proliferation of the mesenteric lymph node cells from acutely infected mice. Reconstitution was dependent on M phi that displayed I-A and I-E subregion-encoded determinants. In higher doses the activated VigGrM phi were more suppressive toward lymphocytic proliferation than were the less activated ModGrM phi. Apparently, the degree of M phi suppressor activity was in direct relationship to the state of M phi maturation/activation. These data indicate that the inflammatory M phi of the schistosome granuloma may be involved in the focal immune accessory/regulatory events that occur within these T cell-mediated lesions.