Effect of Urine Extract on Leucocyte Peroxidase. An Early Laboratory Finding of Avitaminosis B

Tamotsu Suzuki, Akira Takamatsu
1935 Tohoku journal of experimental medicine  
In a preceding paper1) (57th Report of the Peroxidase Reaction) Suzuki, one of the authors, has made an investigation into the rela tion between the urine of mothers with human milk negative to Arakawa's reaction and the prolongation of the shortest proxidase staintime of normal blood neutrophiles, in other words, into the relation between the urine from a B-avitaminotic body and the prolonging ef fect of the B-avitaminotic urine. And he has found out that an appli cation of the urine from
more » ... nts with Arakawa-negative milk causes a prolongation of the shortest peroxidase stain-time of normal blood neutrophiles from a normal person. On the contrary, the urine from mothers with Arakawa-positive milk will not, as lie has found out, cause any prolongation at all. Now wee) know already that an Arakawa-negative milk, that is, milk from a B-avitaminotic body causes the prolongation in ques tion, while no prolongation will occurwith an Arakawa-positive milk. And we know further that, as Takamatsu,3) one of us, has been able to isolate a methyl glyoxal-like substance from human milk negative to Arakawa's reaction, an ether extract of the Arakawa-negative milk will cause the prolongation in question. And synthesized me thyl glyoxal itself does the same thing, as our experiment2) has shown.
doi:10.1620/tjem.25.588 fatcat:e2zpo5zxdfd7bmej3k2mzukixq