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We developed a novel method to observe the released threadlike structure spread on rat internal organs. The technique we used is to spray hematoxylin on internal organs to stain nuclei ofthe threadlike structure. We scrutinized the surface ofinternal organs under stereomicroscope to find the nevel structure. Confocal microscope images revealed the distinctive characteristic pattern ofnuclei distribution that was consistent with the Bonghan's circulatory network,doi:10.18936/islis.22.2_455 fatcat:dqlc6vndnjgp7obv7svjrx7rwy