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Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells in vivo
2016
Guoji Yanke Zazhi
AIM: To study in vivo the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by radioimmunoassay. Break-up time(BUT)of tear film was tested in the different experimental points. Mice corneal epithelia were peeled and MUC1 and MUC4 mRNA and protein levels were measured by RT-PCR and Western blot. RESULTS:
doi:10.3980/j.issn.1672-5123.2016.7.06
doaj:2db5bfbb788a484fbeaab606fd6bb9c6
fatcat:2gmxdwi54ngp7o2n5g6eppm4tu