VDR Pathway-Specific Dose-Related Effects of 1,25(OH)2D3 on Salmonella typhimurium-induced Mouse Colitis [post]

2020 unpublished
Whether and how 1,25(OH) 2 D 3 supplementation influences VDRs in experimental mice with colitis remains to be seen. To explore the effect of 1,25(OH) 2 D 3 on S. typhimurium colitis through the VDR pathway and to discover the role of VDR in its action. Methods: We established a mouse UC model induced by S. typhimurium. After streptococcal typhus infection, the mice were fasted for 12 hours. Blood was collected by the eyeball extraction method, and then sacrificed by cervical dislocation,
more » ... dislocation, specimens were collected for corresponding indicators. Results: Mice exposed to S. typhimurium infection developed signs of acute colitis. After HE staining were performed on the diseased colons from the mice. high dose VD supplementation, the pathological colonic damage did not improve in the mice, and there was no statistical difference between the groups with VD deficiency (P>0.05). VDR expression in the UC group treated with Salmonella was higher than that in the control group, a statistically significant difference (P<0.01). Compared with the VDD+UC group, VDR expression rose in both the LVDS+UC group and the HVDS+UC group, with VDR protein expression being highest after high dose VD supplementation (P<0.01). Compared with the control and the UC groups, the VDR mRNA expression level in the VDD+UC group was significantly higher, and the colon VDR mRNA expression level decreased after active VD supplementation (Fig.7C ). Conclusions: Our data suggest the need for defining the accurate 1,25(OH) 2 D 3 dose limits that induce an anti-inflammatory effect as current data indicate that higher doses would produce an inflammatory response. HE: hematoxylin-eosin RT-PCR: real-time quantitative PCR Declarations Availability of data and materials The datasets herein used and analyzed are available from the corresponding author on reasonable request. Authors' contributions Fubin Qiu and Lijuan Zhang designed the research and developed the overall research plan. Ying Zhang, Chunyan Li and Xiaoyan Zhang conducted the research; Fubin Qiu and Lijuan Zhang analyzed the data and wrote the paper. All authors read and approved the final manuscript. All authors contributed to the critical revision of the manuscript and final approval of the version to be published. All authors agree to be accountable for all aspects of the work by ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
doi:10.21203/rs.2.21661/v1 fatcat:crqfyomiajb43ifdh2suijxz5y