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The engineering of biological components has been facilitated by de novo synthesis of gene-length DNA. Biological engineering at the level of pathways and genomes, however, requires a scalable and cost-effective assembly of DNA molecules that are longer than $10 kb, and this remains a challenge. Here we present the development of pairwise selection assembly (PSA), a process that involves hierarchical construction of long-length DNA through the use of a standard set of components and operations.doi:10.1093/nar/gkq123 pmid:20194119 pmcid:PMC2860126 fatcat:ht6w3yx3urdabkbfqpeugqq3am