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59 KDa phosphoprotein protease from human dentin
Japanese Journal of Oral Biology
It has been reported that several metalloproteases degrade the matrix component in enamel development. However, no reports are available for the dentin matrix protease except collagenase. The purpose of this experiment was to extract and characterize the metalloprotease from dentin. Human dentin powder was immersed in PBS (pH 7.4) for 12 hours at 4°C, and then the extracts were applied on SDS-PAGE and enzymography. The results obtained were as follows: 1. Several proteases were extracted fromdoi:10.2330/joralbiosci1965.33.61 fatcat:ys32p5vsorb3dke2p3vcfepvha