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Comprehensive in silico mutagenesis highlights functionally important residues in proteins
2008
Bioinformatics
Motivation: Mutating residues into alanine (alanine scanning) is one of the fastest experimental means of probing hypotheses about protein function. Alanine scans can reveal functional hot spots, i.e. residues that alter function upon mutation. In vitro mutagenesis is cumbersome and costly: probing all residues in a protein is typically as impossible as substituting by all non-native amino acids. In contrast, such exhaustive mutagenesis is feasible in silico. Results: Previously, we developed
doi:10.1093/bioinformatics/btn268
pmid:18689826
pmcid:PMC2597370
fatcat:s2b3jfyesffg5iupz4y4qdgjri