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Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a proteindoi:10.1107/s2053230x15024619 pmid:26841758 pmcid:PMC4741188 fatcat:yetpk3d7evcxpiy23j7hud2a2u