Protein stability: a crystallographer's perspective

Marc C. Deller, Leopold Kong, Bernhard Rupp
2016 Acta Crystallographica Section F Structural Biology Communications  
Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein
more » ... allographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.
doi:10.1107/s2053230x15024619 pmid:26841758 pmcid:PMC4741188 fatcat:yetpk3d7evcxpiy23j7hud2a2u