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A method has been developed to solubilize insulin receptors from skeletal muscles. Rat hindlimb muscles were rapidly frozen in liquid nitrogen, powdered, extracted with buffered Triton X-100, and partially purified by differential centrifugation followed by wheat germ agglutinin affinity chromatography. The solubilized receptors exhibit typical curvilinear Scatchard plots in insulin binding assays: rapid, Mn 2+ -dependent autophosphorylation of the p-subunit on exposure to insulin as well asdoi:10.2337/diab.33.7.704 pmid:6547396 fatcat:ez7xe4njmjfalbcw6rhg6sxrda