Phosphorylation of Insulin Receptors Solubilized from Rat Skeletal Muscle

C. F. Burant, M. K. Treutelaar, G. E. Landreth, M. G. Buse
1984 Diabetes  
A method has been developed to solubilize insulin receptors from skeletal muscles. Rat hindlimb muscles were rapidly frozen in liquid nitrogen, powdered, extracted with buffered Triton X-100, and partially purified by differential centrifugation followed by wheat germ agglutinin affinity chromatography. The solubilized receptors exhibit typical curvilinear Scatchard plots in insulin binding assays: rapid, Mn 2+ -dependent autophosphorylation of the p-subunit on exposure to insulin as well as
more » ... ulin-stimulated kinase activity toward histone H2B. Furthermore, when intact soleus muscles were incubated in phosphate-depleted medium containing Na 2 H[ 32 P]PO 4 , addition of insulin stimulated the in situ phosphorylation of the p-subunit of the insulin receptor. The ability to rapidly and efficiently isolate insulin receptors from skeletal muscle may permit investigation of factors that modulate insulin action in this tissue. DIABETES 33:704-708, July 1984.
doi:10.2337/diab.33.7.704 pmid:6547396 fatcat:ez7xe4njmjfalbcw6rhg6sxrda