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P < 0.01; liver: P < 0.01; ovary: P < 0.01), while the levels of methane dicarboxylic aldehyde (plasma: P < 0.01; liver: P < 0.001; ovary: P < 0.001) and mRNA expression of mitochondrial transcription factor A (P < 0.001) in granulosa cells were higher. These results indicated that massive ovulation aggravated oxidative stress and had adverse effects on ovarian function in laying hens.doi:10.3906/vet-1603-57 fatcat:m4azm6m6d5bvlpacpxivjtqwxm