P2X1 receptor-mediated pressor responses in the anesthetized mouse

Lu Li, Yi Wu, Mo Deng, Guangyi Wu, Leiming Ren
2012 Acta Pharmaceutica Sinica B  
P2X 1 receptors and adrenoceptors are mainly responsible for vasoconstriction in a variety of blood vessels. However, previous studies have shown that a,b-methylene adenosine 5 0 -triphosphate (a,b-MeATP), a stable analogue of ATP, can induce both pressor and depressor responses in laboratory animals. In this study, the effects of increasing intravenous doses of a,b-MeATP and noradrenaline (NA) (0-30 nmol/kg) administered at 20 min intervals on systolic (SBP), diastolic (DBP) and mean (MBP)
more » ... d pressure in groups of anesthetized mice (n¼ 6) were compared. Both a,b-MeATP and NA caused transient, dose-dependent increases in SBP, DBP and MBP but the effect of a,b-MeATP was more rapid and significantly larger at doses of 10 and 30 nmol/kg (Po0.01). At the dose of 30 nmol/kg, a,b-MeATP increased SBP, DBP and MBP by 65.877.0, 65.775.0 and 65.775.5 mmHg, respectively, compared to increases of 36.874.6, 33.374.9 and 34.574.7 mmHg, respectively, produced by NA. These results indicate P2X 1 receptors play an important role in BP regulation although purinergic vasoconstriction alone may not explain the more potent pressor response to a,b-MeATP in the anesthetized mouse.
doi:10.1016/j.apsb.2012.04.005 fatcat:77cwb4wczbeh7jtnadeijd7hre