In Vivo NMR Micro-Imaging of Kidney and Liver of Mouse at 9.4 T

Mitsuo Kosaka, Nobu Owatari, Yoshiteru Seo, Hidehiko Kawakubo, Seiichi Harada, Tatsuya Katsumata, Hiroaki Ida, Volker Lehmann
2000 The Japanese Journal of Physiology  
The mouse has become a much more common experimental animal for physiological and pathological studies. Compared with rats, the advantages of mouse are its small size, short lifecycle and more pathological models, including a wide variety of transgenic mice, etc. In vivo NMR spectroscopy and imaging are major non-destructive techniques in the physiological and pathological fields [1, 2] . However, the mouse is not as popular as rats for NMR studies. There are only a few reports on the mouse
more » ... ts on the mouse brain [3], heart [4], liver [5] , and kidney [6] . We have applied high-field NMR micro-imaging to the liver and kidney of mice. The contrast of imaging is mainly based on longitudinal relaxation (T 1 ), transverse relaxation (T 2 ), magnetic susceptibility differences (T 2 *) and blood perfusion. Preliminary results were obtained from two pathological models: (1) schistosomiasis Mansoni, and (2) lupus nephritis in an MRL/lpr mouse. From these results, we will present the usefulness of high-field imaging in studying physiological and pathological conditions. Materials and Methods The BALB/C mice (10-20 weeks, 20-30 g) were used as a control. BALB/C mice were infected by a subcutaneous injection of 150 cercaria of schistosoma Mansoni and then followed for 13 weeks. MRL/lpr mice (8-30 weeks) were used as a model for lupus nephritis. All mice were anesthetized by pentobarbital (50 mg/kg I.P.), and were fixed on an animal holder of a mini0.5 imaging probe (Bruker, Germany) in the head-up position. Animal experiments in this report were carried out under the rules and regulations of the Animal Care and Use Committee, School of Medicine, Nagasaki University. In vivo NMR micro-imaging measurements were conducted on a DMX-400wb NMR spectrometer (Bruker, Germany) which consisted of a 9.4 T vertical superconducting magnet with a room temperature bore of 89 mm in inner diameter and a mini0.5 imaging probe (maximum gradient strength 20 G/cm, gradient rise time 300 s and 1 H radio frequency coil of 38 mm in diameter). A micro5.0 imaging probe (maximum gradient strength 100 G/cm, gradient rise time Abstract: In vivo NMR micro-images of the kidney and liver were measured at 9.4 T in intact mouse, schistosoma Mansoni-infected mouse, and lupus nephritis in an MRL/lpr mouse. Highfield NMR imaging has the advantages of sensi-tivity, spatial resolution, and tissue contrasts based on longitudinal and transverse relaxations, magnetic susceptibility differences and blood flow.
doi:10.2170/jjphysiol.50.463 pmid:11082546 fatcat:ltwfnekvxfgibdqhabqcbumeyu