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Leishmania donovani is a unicellular parasite that causes visceral leishmaniasis, a fatal disease in humans. In this study, a complete assembly of the genome of L. donovani is provided. Apart from being the first published genome of this strain (HU3), this constitutes the best assembly for an L. donovani genome attained to date. The use of a combination of sequencing platforms enabled to assemble, without any sequence gap, the 36 chromosomes for this species. Additionally, based on thisdoi:10.1038/s41598-019-42511-4 pmid:30992521 pmcid:PMC6467909 fatcat:lrxrj5bk5nafdeteknmbu7sfuq