Anti-proliferative effect of honokiol in oral squamous cancer through the regulation of specificity protein 1

DONG-WOOK KIM, SEON MI KO, YOUNG-JOO JEON, YOUNG-WOOCK NOH, NAG-JIN CHOI, SUNG-DAE CHO, HONG SEOP MOON, YOUNG SIK CHO, JAE-CHEN SHIN, SEON-MIN PARK, KANG SEOK SEO, JI-YOUNG CHOI (+2 others)
2013 International Journal of Oncology  
Honokiol (HK), a novel plant-derived natural product, is a physiologically activated compound with polyphenolic structure, and has been identified to function as an anticancer agent. It has been widely used in several diseases as a traditional medicine for a long time. We investigated whether HK could show anticancer effects on two oral squamous cell lines (OSCCs), HN-22 and HSC-4. We demonstrated that HK-treated cells showed dramatic reduction in cell growth and apoptotic cell morphologies.
more » ... ll morphologies. Intriguingly, the transcription factor specificity protein 1 (Sp1) was significantly inhibited by HK in a dose-dependent manner. Furthermore, we checked changes in cell cycle regulatory proteins and anti-apoptotic proteins at the molecular level, which are known as Sp1 target genes. The important key regulators in the cell cycle such as p27 and p21 were up-regulated by HK-mediated down-regulation of Sp1, whereas anti-apoptotic proteins including Mcl-1 and survivin were decreased, resulting in caspase-dependent apoptosis. Taken together, results from this study suggest that HK could modulate Sp1 transactivation and induce apoptotic cell death through the regulation of cell cycle and suppression of anti-apoptotic proteins. In addition, HK may be used in cancer prevention and therapies to improve the clinical outcome as an anticancer drug.
doi:10.3892/ijo.2013.2028 pmid:23877711 fatcat:cnvbvirz3rhrhm6rtiqr2dhboa