Potential Molecular Mechanism of Yishen Capsule in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Molecular Docking

Hu Y, Liu S, Liu W, Zhang Z, Liu Y, Li S, Sun D, Zhang G, Fang J
2022 Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy  
Yaling Hu,1,2 Shuang Liu,3 Wenyuan Liu,2 Ziyuan Zhang,2 Yuxiang Liu,1 Sufen Li,2 Dalin Sun,2 Guang Zhang,2 Jingai Fang2 1Shanxi Medical University, Taiyuan, Shanxi, 030001, People's Republic of China; 2Department of Nephrology, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, People's Republic of China; 3Department of Urology, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, People's Republic of ChinaCorrespondence: Jingai Fang, Department of
more » ... First Hospital of Shanxi Medical University, 85 Jiefangnan Road, Taiyuan, Shanxi, 030001, People's Republic of China, Email jingaifang2017003@126.comPurpose: Using network pharmacology and molecular docking to explore the mechanism of Yishen Capsule in the treatment of diabetic nephropathy.Materials and Methods: Active components of Yishen Capsule were obtained using database such as TCMSP and TCMID. UniProt protein database was used to screen and standardize the human-derived targets of the active chemical components. Diabetic nephropathy (DN) targets were obtained from databases such as GeneCards, OMIM, TTD, DisGeNET and DrugBank. A network of "Yishen Capsule Components-diabetic nephropathy Targets-Pathways" was constructed by analyzing data above to screening out core targets for molecular docking verification. DN is induced by streptozocin in rats after left nephrectomy. Renal tubular epithelial cells (RTECs) was isolated and cultured under high glucose conditions. Based on these experimental models, key pathway target genes screened by network pharmacology were verified both in vitro and in vivo.Results: The main active components of Yishen Capsule in the treatment of DN include quercetin, kaempferol, gallic acid, astragaloside IV, etc. Some key targets (such as AR, AKT1, TP53, ESR1, JUN) and important signal pathways (such as AGE-RAGE, HIF-1 and JAK-STAT signal pathway) were included in the treatment of DN with Yishen Capsule. Molecular docking assay showed that most of the targets have good binding activity with t [...]
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