Rapid low dose electron tomography using a direct electron detection camera

Vadim Migunov, Henning Ryll, Xiaodong Zhuge, Martin Simson, Lothar Strüder, K. Joost Batenburg, Lothar Houben, Rafal E. Dunin-Borkowski
2015 Scientific Reports  
We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15-60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose
more » ... ctron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution.
doi:10.1038/srep14516 pmid:26434767 pmcid:PMC4592966 fatcat:4p42sillfzb3vk3gwcyy5tya6e