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Isolation, screening, characterization of proteolytic bacteria and production of protease with its potential applications
Pure and Applied Biology
The purpose of this study was production of protease enzyme by isolated bacteria and evaluation of their potential for various industrial applications. Two strains AN16 and AN13 exhibiting maximum proteolytic activity were used for further studies. The activity of crude enzymes was completely inhibited by phenyl-methyl-sulphobnyl fluoride (PMSF) and ethylene diamine tetraacetic acid (EDTA) had no such negative effect on activity. Enzymes were relatively stable in presence of surface activedoi:10.19045/bspab.2020.90240 fatcat:vnqj4cni75gdhik2q7unbeuhsq