Interactions of polydispersed single-walled carbon nanotubes with T cells resulting in downregulation of allogeneic CTL responsesin vitroandin vivo

Anwar Alam, Sumedha Sachar, Niti Puri, Rajiv K. Saxena
2012 Nanotoxicology  
Addition of polydispersed acid functionalised single-walled carbon nanotubes (AF-SWCNTs) significantly suppressed alloimmune cytotoxic T cell (CTL) response generated in a mixed lymphocyte reaction (MLR) between spleen cells from C57BL/ 6 (H-2 b ) and BALB/c (H-2 d ) mice. AF-SWCNTs treatment also decreased CD69 expression, enhanced apoptotic response in T cells and reduced significantly the recovery of live CD4 + and CD8 + T cells from MLR cultures. A two to threefold increase was noticed in
more » ... e binding/uptake of AF-SWCNTs by T cells in MLR cultures as compared with control cultured T cells. Confocal microscopy confirmed the internalization of AF-SWCNTs by live CD8 + T cells in MLR cultures. Administration of AF-SWCNTs suppressed the generation of anti-P815 CTL response in C57BL/ 6 mice and the recovery of T-cell populations from the spleens. The results demonstrate a suppressive effect of AF-SWCNTs on CTL response and provide an insight into the mechanism of this suppression.
doi:10.3109/17435390.2012.739666 pmid:23057773 fatcat:2xuqpl6a3jf2ndaejnliwva4aq