Autocrine Growth Factor Production by Fetal, Keloid, and Normal Dermal Fibroblasts
Archives of Facial Plastic Surgery
Objective: To evaluate differences in fibroblast autocrine growth factor production by human fetal, keloid, and normal adult dermal fibroblasts. Design: Serum-free cell lines of fetal, keloid, and normal adult dermal fibroblasts were established. Cell counts were performed and supernatants collected at 4, 24, and 72 hours. Cell-free supernatants were quantitatively assayed for transforming growth factor ␤1 (TGF-␤1) and basic fibroblast growth factor (bFGF). Results: Population doubling times
... n doubling times for fetal, keloid, and normal adult fibroblasts were 120.0, 88.1, and 128.4 hours, respectively. Differences in population doubling times did not reach statistical significance. Statistically significant differences between TGF-␤1 levels from fetal and normal adult fibroblasts were seen at 24 and 72 hours. Significant differences between TGF-␤1 levels from keloid and normal adult fibroblasts were also seen at 24 and 72 hours. Fetal fibroblasts demonstrated higher levels of bFGF than normal adult fibroblasts at each time point, but these differences were not statistically significant. No significant differences were observed between keloid and normal adult bFGF levels.