B. Doskaliuk, L. Zaiats, R. Yatsyshyn
2021 Annals of the Rheumatic Diseases  
Background:Systemic sclerosis (SSc) is a heterogeneous autoimmune pathology, which detailed pathogenetic mechanisms of initiation and progression still require further investigations. It could be achieved with implementation of reliable experimental modeling techniques.Objectives:This study was aimed to determine the state of oxidant/antioxidant system, dysregulation of collagen synthesis in laboratory animals and evaluate effectiveness of the considered model of SSc induction.Methods:Thirty
more » ... lt Wistar rats (220-240 g) were included in the study. The experimental group consisted of 20 animals, which received 3 subcutaneous injections of 0.5 ml of 5% sodium hypochlorite solution (NaClO) with an active chlorine concentration of 190 g / dm3 for 6 consecutive weeks. The control group consisted of 10 rats injected with isotonic solution according to the previously described scheme.Results:The level of catalase in the experimental group was lower than in the control 9.69 ± 0.67 g / l and 11.1 ± 0.87 g / l respectively (p = 0.002). Protein oxidative modification (POM) products were higher in the experimental group (0.0722 ± 0.023 vs 0.0527 ± 0.023, p = 0.014). Malondialdehyde (MDA) was also higher in the experimental group compared to the control (4.30 ± 0.26 and 3.85 ± 0.23, respectively, p = 0.010). The concentration of oxyproline in the serum of laboratory animals subjected to repeated administration of NaClO was significantly higher compared to the control group (34.03 ± 5.01 and 33.23 ± 2.40, p = 0.020).Conclusion:As a result of the use of NaClO for the reproduction of SSc in laboratory animals, there is a violation of oxidative-antioxidant homeostasis and increased fibrotic processes. These changes are comparable with those in SSc patients, which makes this modeling technique a promising tool for a detailed investigation of SSc pathogenesis.Disclosure of Interests:None declared
doi:10.1136/annrheumdis-2021-eular.732 fatcat:tjoascabx5dm7mzfflf2er7lwq