MOESM1 of Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis

Chaoyang Zhou, Bin Ye, Shan Cheng, Leizhen Zhao, Yuanxin Liu, Jiandong Jiang, Xin Yan
2019 Figshare  
Additional file 1. Fig. S1–S2 The expression pattern of BgaB in mutant strains with different promoters. Fig. S3–S4 The expression pattern of BgaB controlled by mutant Pylb. Fig. S5-S6 The expression pattern of BgaB controlled by mutant P3510. Fig. S7–S8 The expression pattern of sfGFP in mutant strains. Fig. S9–S10 The control of MPH (a) and Chd (b) expression in strain WB800. Fig. S11 Purification of BgaB and sfGFP. Fig. S12 Purification of MPH and Chd. Fig. S13 Verification of BgaB, sfGFP,
more » ... H and Chd by western blot. Fig. S14-S16 The kinetics of bacterial growth. Fig. S17 The kinetic model for BgaB production in mutant strains. Table S1 Primers used in this study. Table S2 the parameters in the kinetics of bacterial growth. Table S3 the parameters in the kinetics of BgaB production.
doi:10.6084/m9.figshare.8280374.v1 fatcat:feeakslorbbcjjhvsospnemhyu