Poster Sessions

2007 Annals of Nutrition and Metabolism  
Room 656, teaching and research building, 250 Wu-Xin Street, Taipei City, Taiwan Previously, we have shown that indole-3-carbinol (I3C), a compound derived from cruciferous vegetable, inhibits different inflammatory mediators in lipopolysaccharide (LPS)-activated macrophages and suppresses angiogenic markers in vascular endothelial cells. Because the activated macrophages may release angiogenic factors to induce angiogenesis, the aim of this study was to investigate the effects I3C on activated
more » ... macrophage-induced angiogenesis. In cocultivated macrophages and vascular endothelial cells, LPS significantly enhanced nitric oxide (NO) production and tube formation, whereas cotreatment with I3C inhibited such enhancement. To clarify the roles of I3C on macrophages, the macrophages were treated with LPS as well as I3C or NO synthase inhibitor, Nitro-L-arginine methyl ester (L-NAME), and the treated condition medium (CM) was used to cultivate vascular endothelial cells. The results indicated that I3C or L-NAME containing CM significantly inhibited CM-stimulated tube formation, vascular endothelial growth factor (VEGF) secretion, and matrix metalloproteinase-9 (MMP-9) activity. To understand the effects of I3C or L-NAME on vascular endothelial cells, the LPSactivated macrophage CM was used to cultivate vascular endothelial cells in the presence of I3C or L-NAME. The results showed that I3C and L-NAME inhibited CM-induced NO production, tube formation, VEGF secretion and, MMP-9 activity in vascular endothelial cells. In summary, we demonstrate that the cruciferous vegetable derivative, I3C, not only inhibits LPS stimulated macrophage activation but also affects vascular endothelial cells to inhibit macrophage-induced angiogenesis, and such effect is dependent upon NO production.
doi:10.1159/000105121 fatcat:7tiintkunjcjtcmhez2nxndjve