PPV-Based Conjugated Polymer Nanoparticles as a Versatile Bioimaging Probe: A Closer Look at the Inherent Optical Properties and NanoparticleCell Interactions [component]

unpublished
The molecular weight distribution of MDMO-PPV and CPM-PPV-co-MDMO-PPV were measured via a Tosoh EcoSEC (Tessenderlo, Belgium) system HLC-8320GPC, comprising an autosampler, PSS guard column SDV of 50 x 7.5 mm, followed by 3 PSS SDV analytical linear XL columns of 300 x 7.5 mm (5µm) at 40 °C (column molecular weight range is 1 x 10 2 -1 x 10 6 g·mol -1 ) and a differential refractive index detector, Tosoh EcoSEC RI, using THF as eluent with a flow rate of 1 mL*min -1 . The used flow marker was
more » ... luene and calibration was performed using linear narrow polystyrene standards of 470 -7.5 x 10 6 g·mol -1 obtained from PSS Laboratories (Valkenburg, The Netherlands) (MHKS for precursor (α = 0.67605 and k = 0.000142 ml·g-1) as well as conjugated MDMO-PPV (α = 0.809 and k = 0.00002 ml·g -1 ) were applied for molecular weight analysis). To determine the copolymer composition of CPM-PPV-co-MDMO-PPV, 1 H-NMR spectra were recorded in CDCl3 on a Varian Inova 300 spectrometer (Chemnitz, Germany) (300 MHz and 75MHz respectively, 5 mm probe). ATR-IR spectra were collected with a Bruker (Brussels, Belgium) Tensor 27 FT-IR spectrophotometer. The study of both size and morphology of the conjugated nanoparticles was performed with TEM on a Tecnai G2 spirit twin, FEI (Zaventem, Belgium), at an accelerating voltage of 120 keV. TEM images were processed with ImageJ software. The respective samples were drop casted and dried on copper grids. In parallel, the hydrodynamic diameter and polydispersity as well as zeta-potential were determined with dynamic light scattering using ZetaPALS equipment, Brookhaven Instrument Cooperation (Waddinxveen, The Netherlands). The solid content of all samples was determined by thermogravimetric analysis.
doi:10.1021/acs.biomac.6b00574.s002 fatcat:qtokciq6krf6nkt556pjxeotn4