PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers

William A. Walters, J. Gregory Caporaso, Christian L. Lauber, Donna Berg-Lyons, Noah Fierer, Rob Knight
2011 Computer applications in the biosciences : CABIOS  
Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task. Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data. Availability: PrimerProspector is open-source software available at
doi:10.1093/bioinformatics/btr087 pmid:21349862 pmcid:PMC3072552 fatcat:5hjzj2h6wfccxanexj2mkyksse